Coding

Part:BBa_K1916009

Designed by: Jonathan Chen   Group: iGEM16_UC_Davis   (2016-10-11)


Osc7112_5903g3 + intein-CBD tag

A GAF domain of the cyanobacteriochrome (CBCR) Osc7112_5903 with an intein/chitin-binding domain attached to the C-terminus for purification. As with most CBCRs, NpF requires a phycocyanobilin co-factor, and must therefore be expressed in a system with heme oxygenase (BBa_I15008) and PcyA (BBa_I15009). RFC10 compatible.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 589
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 636
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1156
    Illegal AgeI site found at 299
    Illegal AgeI site found at 1246
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None